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. 2004 Nov;124(5):527–540. doi: 10.1085/jgp.200409153

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Copyright © 2004, The Rockefeller University Press

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Figure 4. — Ionomycin treatment induces phosphorylation and dephosphorylation of the same polypeptides that are modified by extracellular ATP. Typical pattern of phosphorylated polypeptides in cilia isolated from epithelial tissue after exposure to ionomycin (1 μM) for 0.5 min (lane 2) or 1 min (lane 3), as compared with untreated control (lane 1). Western blot analysis using anti-phosphoserine antibody indicates an increase in phosphorylation of 27, 37, and 44 kD polypeptides, and a decrease in phosphorylation of 35, 69, 100, and 130 kD polypeptides. A representative blot of eight similar experiments is shown.