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. 2004 Nov;124(5):527–540. doi: 10.1085/jgp.200409153

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — Inhibition of NOS abolishes extracellular ATP-dependent phosphorylation (A) and dephosphorylation (B) of ciliary polypeptides in intact tissue. Epithelial tissue was preincubated for 10 min with or without 50 μM L-NAME followed by stimulation with extracellular ATP (100 μM) for 5 min. Cilia were then isolated and subjected to Western blot analysis, as described under materials and methods. Quantitative analysis of the magnitude of phosphorylation shows the complete elimination of phosphorylation and dephosphorylation induced by ATP (100 μM) in the presence of L-NAME (50 μM). Each column represents the mean ± SEM averaged over six experiments.