TABLE II.
Conductance–Voltage Data for HCN2 Channels
| Control | Saturating cAMP | ||||
|---|---|---|---|---|---|
| HCN2 channel | Vhalf (mV) | Slope (mV) | Vhalf (mV) | Slope (mV) | ΔVhalf (mV) |
| Wild type (7) | −125 ± 2.0 | 3.76 ± 0.30 | −111 ± 1.7 | 4.18 ± 0.24 | 14.3 ± 1.8 |
| K472E (4) | −113 ± 3.1 | 9.59 ± 0.89 | −112 ± 2.9 | 9.06 ± 0.91 | 0.75 ± 1.3 |
| E502K (6) | −128 ± 2.9 | 5.66 ± 0.97 | −116 ± 2.8 | 5.4 ± 1.1 | 12.3 ± 0.94 |
| K472E + E502K (4) | −125 ± 3.8 | 5.55 ± 0.82 | −110 ± 3.4 | 5.72 ± 0.41 | 14.9 ± 1.6 |
| D542K (5) | −124 ± 1.9 | 7.03 ± 0.61 | −114 ± 1.9 | 6.35 ± 0.75 | 10.1 ± 1.4 |
| K472E + D542K (3) | −133 ± 1.9 | 3.65 ± 0.57 | −119 ± 3.6 | 3.52 ± 0.31 | 14.2 ± 1.9 |
| E502K + D542K (4) | −118 ± 2.8 | 7.58 ± 1.4 | −122 ± 2.8 | 7.27 ± 1.1 | −3.9 ± 0.95 |
All values are mean ± SEM. The n values are in parentheses after each channel construct. ΔVhalf = Vhalf cAMP − Vhalf control.