Figure 3.

siRNA functional profiling in CMK cells. CMK cells (10⁵) were suspended in siPORT buffer and incubated with 1 μM siRNA from an siRNA library individually targeting each member of the tyrosine kinase family as well as N-RAS, K-RAS, and single and pooled nonspecific siRNA controls. Cells were electroporated on a 96-well electroporation plate at 2220 V (equivalent of 300 V), 100 μsec, 2 pulses. Cells were replated into culture media and cell viability and proliferation was determined by an MTS assay at day 4 after electroporation. Values represent percent mean (normalized to nonspecific control wells) plus or minus SEM (n = 3).