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. 2005 Jun;125(6):535–553. doi: 10.1085/jgp.200409216

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Copyright © 2005, The Rockefeller University Press

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Figure 1. — The localization of SMCs and the contractile responses of an airway and arteriole in a lung slice induced by ACH, 5-HT, and KCl. (A) A lung slice was fixed and stained with FITC-conjugated antibodies against smooth muscle α-actin. Fluorescence and phase-contrast images were recorded with a confocal microscope. The fluorescence image was assigned a green pseudo-color and superimposed on the phase-contrast image to show colocalization. The final image consists of a montage of six different images. The bronchiole airway (A) and the accompanying arteriole (a) are surrounded by alveolar parenchyma. A prominent layer of SMCs (green) is located below the epithelial (EPC) and endothelial (ENC) cells of the bronchiole and arteriole, respectively. (B) A series of phase-contrast images, recorded at different times (indicated by arrows in C) showing the appearance of an airway and arteriole before (1) and after stimulation with 1 μM ACH (2), 1 μM 5-HT (3), and 100 mM KCl (4). (C) The cross-sectional area of the lumen of an airway (blue line) and arteriole (red line) with respect to time in response to ACH, 5-HT, and KCl (top bars). ACH induced a contraction of the airway but not of the arteriole. 5-HT induced a greater contraction in the arteriole than in the airway. KCl induced twitching in both the airway and arteriole and a sustained contraction of the arteriole. Upon washout of agonists or KCl, the airway and arteriole relaxed. A movie of these data is shown in Video 1 (available at http://www.jgp.org/cgi/content/full/jgp.200409216/DC1). Representative experiment of six different slices from three mice.