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Lung slice with preserved architecture. The companion manuscripts by Perez and Sanderson (2005a)(b) describe the use of 100 μm lung sections cut on a vibratome after the airspaces and pulmonary arteries were separately filled. Agarose remains in the alveolar spaces, maintaining the distension of the lung parenchyma. Shown are paired bronchioles and pulmonary arterioles, as well as neural control elements.
