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. 1999 Mar 30;96(7):3606–3610. doi: 10.1073/pnas.96.7.3606

Figure 5.

Figure 5

Mapping of the binding site of aptamers TR-D20, TR-D28, and TR-D31 and the negative control sequence TR-D42 on CD18cyt by using synthetic biotinylated peptide fragments. All gel shifts were carried out in the presence of 25 μM streptavidin to enhance separation of shifted vs. nonshifted RNA. Gel-shift experiments were performed in the presence of 4 nM radiolabeled RNA and 40 μM nonspecific tRNA competitor. Lane 1, free aptamer in the presence of 25 μM streptavidin and 40 μM unspecific competitor tRNA; lanes 2, 4, and 6 or 3, 5, and 7, same as lane 1 in presence of 25 μM or 12.5 μM, respectively, peptides A23 (lanes 2 and 3), B16 (lanes 4 and 5), and C17 (lanes 6 and 7).