Fig. 1.

Schematic diagram of EbolaΔVP30 constructs. The top row shows a schematic diagram of the EBOV genome flanked by the leader sequence (l) and the trailer sequence (t) in positive-sense orientation. Two unique restriction sites for SalI and SacI (positions 6180 and 10942 of the viral antigenome, respectively) allowed the subcloning of a fragment that spans the VP30 gene. The subgenomic fragment was then used to replace the VP30 gene with genes encoding neo or eGFP, respectively. By using the unique restriction sites, the altered subgenomic fragments were cloned back into the full-length EBOV cDNA construct.