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. 2008 Jan 22;105(4):1129–1133. doi: 10.1073/pnas.0708057105

Fig. 3.

Fig. 3.

Replication kinetics of wild-type EBOV and EbolaΔVP30-neo virus. VeroVP30 cells (Upper) and wild-type Vero cells (Lower) were infected with EBOV or EbolaΔVP30-neo at a high m.o.i. of 1.0 (Left) or a low m.o.i. of 0.01 (Right). Supernatants were harvested every 24 h after infection for 6 days. Viral titers of the respective viruses were determined by infecting confluent VeroVP30 cells or wild-type Vero cells with 10-fold dilutions of the supernatants and subsequent immunostaining as described in Materials and Methods. Virus titers for EbolaΔVP30-neo virus (filled squares) and wild-type EBOV (open circles) were comparable in VeroVP30 cells (Upper). In wild-type Vero cells (Lower), no replication was detected for EbolaΔVP30-neo virus (filled squares).