Fig. 4.
R197 substitutions can affect Ig light chain folding. COS cells were transfected with the indicated BiP constructs along with mouse λ light chains. Cells were pulse-labeled with [35S]methionine in the presence of DTT (0) and then chased in complete media without DTT for 45 min. Cell lysates were immunoprecipitated with anti-λ antibodies, and proteins were electrophoresed under nonreducing conditions. The migration of completely reduced, partially oxidized, and completely oxidized light chains is indicated at the right.