Fig. 2.
IKKγK270A dominantly controls NF-κB activity. (A) Helical wheel representation of CC2. Beginning (position 253) and end (position 288) of CC2 and the occurrence of K270 in a “d” position are indicated. (B) NF-κB-dependent GFP induction measured 48 h after the transduction of GTPT3 cells with a control gene (bsd) or the indicated IKKγ alleles. Cells were stimulated with or without 1 μg/ml LPS for the final 24 h as indicated. An IRES-controlled CD8 was used to distinguish transduced from nontransduced cells. (C) F40 cells containing NF-κB-dependent luciferase were transduced with IKKγ, either wild type or mutant in position 270, and carrying additional mutations as indicated. Luciferase activity was measured 24 h later. Lysates were probed for AU1-tagged IKKγ.