Fig. 4.

Emergence by EEG and EMG criteria in wild-type mice while orexin/ataxin-3 siblings remain anesthetized. At 9.8 ± 2.2 min after discontinuation of the isoflurane, wild-type mice exhibit EEG and EMG evidence of emergence from anesthesia as depicted by the top two tracings that show the abrupt return of low-amplitude, fast-frequency EEG (tracing 1), along with concurrent EMG tone (tracing 2). Emergence occurs with a similar appearance in orexin/ataxin-3 mice but is significantly delayed at 19.2 ± 2.9 min.