Fig. 3.

Mast cells are closely apposed to smooth muscle cells expressing AT₁R in rat bronchus. (A–C) Rat bronchial mast cell colabeled with avidin-FITC (A) and an anti-renin Ab (B). Color overlay is shown in C with the nuclear stain, DAPI (blue). (Scale bar, 5 μm.) (D) Representative section of rat bronchus costained with avidin-rhodamine (to identify mast cells, red) and an anti-AT₁R Ab conjugated to Alexa Fluor 488. Smooth muscle cells stained positive for AT₁R (green). (Scale bar, 15 μm.) (E) Toluidine blue-stained mast cells adjacent to smooth muscle cells in rat bronchus. (Scale bar, 30 μm.)