Table 1.
Recovery parameters after CTX-Clv application
| Construct | Treatment | τrecovery, s | % recovery | n |
|---|---|---|---|---|
| Q1/E1 | CTX-Clv | 98 ± 1 | 95 ± 2 | 5 |
| Q1/E1T14C | CTX-Clv, TCEP | 97 ± 1 | 96 ± 2 | 4 |
| Q1/E1T14C | CTX-Clv + excess CTX | 155 ± 4 | 96 ± 1 | 3 |
| Q1/E1T14C | CTX-Clv, CTX-Mal, TCEP | 94 ± 3 | 94 ± 1 | 3 |
| Q1/E1T14C | Round 1, TCEP | 96 ± 2 | 96 ± 2 | 3 |
| Q1/E1T14C | Round 2, TCEP | 98 ± 3 | 98 ± 4 | 3 |
| Q1/E1T14C | Round 3 | 97 ± 1 | 94 ± 3 | 3 |
Data are from individual experiments obtained from 3–5 oocytes. Treatment describes the sequential application of chemical reagents to the indicated construct. Round 1, Round 2, and Round 3 indicates the first, second, and third rounds of subunit counting (Fig. 4a), respectively. Time constants of recovery (τrecovery) were fit to a single exponential as described in Materials and Methods. Percent recovery is a comparison between the amount of current remaining after CTX-Clv release with the amount of current before CTX-Clv application. Values are mean ± SEM.