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. 2008 Jan 24;105(5):1528–1532. doi: 10.1073/pnas.0711357105

Fig. 3.

Fig. 3.

Bit1 KO MEFs are resistant to anoikis. (A) MEFs from wild-type (WT) and KO mice were grown in suspension, harvested, stained with annexin/propidium iodide, and analyzed by flow cytometry at the indicated time points. The percentage of viable cells is shown. The graphs represent MEFs isolated from at least two different WT and KO mice. (B) MEFs from WT and KO mice were treated with 0.2 μM staurosporine for 24 h. Cell death was analyzed as in A by flow cytometry (Left). Cell proliferation (Right) was assessed by using the MTS cell proliferation assay. (C and D) Bit1 KO mice display increased Erk activation. (C) Lysates were made from cultured MEFs of WT, heterozygous (HET), and KO mice. The lysates were analyzed by SDS/PAGE and immunoblotted for total and phosphorylated (active) Erk 1/2 (Thr-202/Tyr-204) and for Bit1. GAPDH immunoblotting was used as a loading control. (D) Lysates from mouse tissues were analyzed as in C.