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. 2008 Jan 25;105(5):1686–1691. doi: 10.1073/pnas.0701675105

Fig. 4.

Fig. 4.

Genetic requirements for NKG2D ligand induction. (A) Staining of lymphomas derived from the indicated genotypes as in Fig. 1A. (B) Staining of lymphoma (Eμ-Myc;Ink4a/Arf+/− 17) and PCR of genomic DNA from lymph node cells derived from same animal or indicated genotypes, assaying for LOH at exon 2. (C) Staining of splenocytes from an early Eμ-Myc;Ink4a/Arf+/− animal. High- and low-FSC gates are constructed within a total live gate as indicated. Numbers reflect the percentage of cells within the live gate that fall within these two populations. Data are representative of more than five experiments. (D) A schematic of the Ink4a/Arf locus (37) with primers specific for exon 1α and exon 2 is shown with quantitative PCR results. Exon 1α was used as a normalizer for genomic copy number among different samples. Each data point represents an individual tumor from a unique mouse. Quantitative PCR is from genomic DNA derived from B cells of the indicated organismal genotype. (E) VDJ usage was analyzed by multiplex PCR and resolved on a sequencer. (Left) Pattern of peaks represents a polyclonal population. (Right) High-FSC B cells from early time points in Eμ-Myc;Ink4a/Arf+/− lymphomagenesis show a largely clonal or oligoclonal profile. Fragment size is plotted on the x axis, and fluorescence intensity is plotted on the y axis.