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. 2008 Feb 11;180(3):537–548. doi: 10.1083/jcb.200705085

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Copyright © 2008, The Rockefeller University Press

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Figure 7. — The dynamics of centrosome migration in akt embryos. (A) Purpose designed automated tracking software was used to identify and follow centrosome movement in wild-type or akt embryos. Data were obtained from 26 wild-type and 16 akt1^q/akt1⁰⁴²²⁶ centrosomal pairs. Error bars show the SEM based on one standard deviation. (B) Stills from time-lapse videos of cycle 11 wild-type (wt) and akt1^q/akt1⁰⁴²²⁶ (akt) embryos. In the akt embryo, a centrosome that appears to have detached from the nuclear envelope does not initially contribute to spindle formation (arrows). The bent, short spindle formed recaptures the centrosome and is capable of chromosome segregation. Nonetheless, the resulting nuclei move into the interior of the embryo during the following interphase. See Videos 9 and 10 (available at http://www.jcb.org/cgi/content/full/jcb.200705085/DC1). Bar, 10 μm.