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. 2008 Feb 11;180(3):459–466. doi: 10.1083/jcb.200711031

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Copyright © 2008, The Rockefeller University Press

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Figure 3. — Microtubule-destabilizing/stabilizing drugs affect meiosis I spindle length and orientation. (A and C) Spindle length and pole depth from the chorion were estimated from z sections (0.5-μm steps) of fixed whole-mount klp10A-gfp (A) or klp10A NT–-gfp oocytes (C) either untreated or treated with colchicine or taxol. Mean ± SEM (error bars). (B) Schematic diagram illustrating the effects of colchicine and taxol on spindle length, angle, and depth relative to the cortex in klp10A-gfp oocytes. Both drugs cause the spindle to move deeper into the oocyte and orient more vertically to the cortex.