Table 6.
PCR primers and conditions for identification of SNPs in TLR2.
| Exon | Primer name | Sequence, 5' → 3' | Tann, °C | Amplicon size, bp |
| TLR2 PROMOTER | ||||
| Part 1 | Forward | CCCTTGAATGTATTTGTCACTTCC | 60 | 664 |
| Reverse | TCCATATTTTTGAGAATCTGACTGA | |||
| Part 2 | Forward | TGCAAGGATTACAGCAGATTTTC | 59 | 579 |
| Reverse | AAAATCATGTATTTACCCACAACG | |||
| Part 3 | Forward | CGTACAAAGATGTGCCAGAGG | 59 | 586 |
| Reverse | CCAGTGTTCTTGCCTGGAGA | |||
| Part 4 | Forward | GGGGCCTTTAATGAGACCTG | 60 | 445 |
| Reverse | GCCCTTCCTAAATTGAGATCAGA | |||
| Part 5 | Forward | GTGCCTTTGCAATGAGGACT | 60 | 496 |
| Reverse | GCCTGAAGAGCAGATGAGGA | |||
| TLR2 GENE | ||||
| Part 1 | Forward | GCCATGATGTCAAACACAGTCA | 58 | 613 |
| Reverse | AGCACTGATCTCAAGCTCCTCA | |||
| Part 2 | Forward | CAAAACACTTGGGGAAACATCT | 59 | 611 |
| Reverse | TTTTAACTCTGCCTGTGAGTGGA | |||
| Part 3 | Forward | ACTGTACCCATGATGGAATTGG | 61 | 643 |
| Reverse | ACGGGTAAGAAGGAGGCATCT | |||
| Part 4 | Forward | CACAGTTTAACCCAGTGCCTTC | 59 | 633 |
| Reverse | CATGAGGTTCTCCACCCAGTAG | |||
| Part 5 | Forward | CGGACTGTGGTACATGAAGATG | 60 | 643 |
| Reverse | TCAGCATCAGTTCTTCCAATGA | |||