Figure 7.

Phylogeny of crustacean metallothioneins. Neighbor-Joining tree constructed from an amino acid alignment of the three Daphnia pulex metallothionein proteins plus 18 sequences from 11 selected insect and crustacean taxa using the E-INS-I algorithm implemented by MAFFT [92]. Gaps within alignments were ignored in pairwise comparisons of the sequences and the genetic distances were corrected by the Poisson distribution model. The NCBI accession numbers are listed beside each taxonomic gene designation. Percent bootstrap support for nodes are shown, which are derived from 1000 pseudo-replication of the data. The Daphnia sequences were deposited at GenBank under the accession numbers Dpu Mtn1 [GenBank:EU307302]; Dpu Mtn2 [GenBank:EU307303]; and Dpu Mtn3 [GenBank:EU307304].