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. 2007 Dec 21;8:477. doi: 10.1186/1471-2164-8-477

Table 2.

Cadmium regulated microarray elements

Up-regulated elements
Siga Seqb EST Cluster M averagec A averagec Hit Description P Valued Putative GO annotations
8 9 Contig 262 1.02 ± 0.31 10.12 ± 1.88 2-domain hemoglobin protein subunit < 0.001 GO:0015671, GO:0005833, GO:0005344
2 16 Contig 272 0.43 ± 0.21 9.06 ± 1.50 CG30045-PA < 0.001 GO:0042302
1 1 Singlet 433 0.71 12.08 CG6305-PA 0.062 GO:0042302
1 1 Singlet 498 1.02 9.15 chitinase-1 0.028 GO:0008061, GO:0006030, GO:0004568, GO:0005576
3 4 Contig 213 0.82 ± 0.12 9.07 ± 0.53 chitinase-2 < 0.001 GO:0008061, GO:0006030, GO:0004568, GO:0005576
1 3 Contig 218 0.93 ± 0.19 9.64 ± 1.77 chitotriosidase 0.005 GO:0008061, GO:0006030, GO:0016798, GO:0005576
1 3 Contig 209 0.41 ± 0.17 12.21 ± 1.48 CUO6 BLACRCuticle protein 6 (BcNCP14.9) 0.06 GO:0042302
4 6 Contig 241 1.07 ± 0.31 10.76 ± 2.07 cuticle protein-1 0.018 GO:0042302
5 8 Contig 257 1.03 ± 0.33 9.96 ± 1.54 cuticle protein-2 < 0.001 GO:0042302
13 28 Contig 273 0.91 ± 0.32 10.05 ± 1.65 cuticle protein-3 < 0.001 GO:0042302
1 3 Contig 149 0.72 ± 0.28 8.55 ± 0.92 cuticle protein-4 0.011 GO:0042302
5 8 Contig 261 0.88 ± 0.30 9.87 ± 1.42 cuticle protein-5 < 0.001 GO:0042302
1 1 Singlet 15 1.23 14.06 ERGA6350 0.009
1 1 Singlet 469 0.80 7.99 helix-loop-helix transcription factor 0.055 GO:0006355, GO:0003677
1 1 Contig 21 1.45 10.03 hemoglobin-1 0.003 GO:0015671, GO:0005833, GO:0005344
1 1 Contig 16 1.08 9.81 hemoglobin-2 < 0.001 GO:0015671, GO:0005833, GO:0005344
1 1 Singlet 65 2.16 8.34 Hypothetical protein CBG14247 < 0.001
4 4 Contig 221 1.98 ± 0.47 8.88 ± 0.82 Metallothionein < 0.001 GO:0046872, GO:0006875
1 5 Contig 220 0.31 ± 0.49 8.95 ± 0.62 myosin 2 light chain 0.08 GO:0005509
2 4 Contig 227 0.70 ± 0.04 12.59 ± 2.37 OPSC1 HEMSACompound eye opsin BCRH1 0.036 GO:0007602, GO:0016021, GO:0001584, GO:0007186
1 1 Singlet 251 1.18 10.90 PREDICTED: similar to chitinase 0.019 GO:0008061, GO:0006030, GO:0016798, GO:0005576
1 1 Singlet 459 1.15 7.42 PREDICTED: similar to glutathione S-transferase 0.021 GO:0016740
1 1 Singlet 1 1.94 8.99 Unknown EST-1 0.009
3 3 Contig 232 1.14 ± 037 8.27 ± 0.99 Unknown EST-2 < 0.001
7 8 Contig 253 1.05 ± 0.44 9.99 ± 1.38 Unknown EST-3 < 0.001
1 1 Singlet 166 0.87 8.20 Unknown EST-4 0.048
1 1 Singlet 64 0.75 9.95 Unknown EST-5 0.059
1 1 Singlet 49 0.74 8.77 Unknown EST-6 0.067
1 5 Contig 237 0.33 ± 0.21 10.11 ± 1.55 Unknown EST-7 0.073
1 1 Singlet 172 0.68 8.23 Unknown EST-8 0.071
24 Unsequenced

Down-regulated elements
Siga Seqb EST Cluster M averagec A averagec Hit Description P Valued Putative GO annotations

1 2 Contig 135 -0.36 ± 0.66 9.28 ± 0.08 carboxypeptidase A1 0.068 GO:0006508, GO:0004182
1 1 Singlet 97 -1.04 6.94 endo-1,4-mannanase 0.009 GO:0016985, GO:0000272
1 3 Contig 202 -0.38 ± 0.36 8.39 ± 1.02 PREDICTED: similar to CG31997-PA 0.036
2 2 Contig 83 -1.29 ± 0.06 8.00 ± 0.40 PREDICTED: similar to monooxygenase 0.001
1 3 Contig 162 -0.37 ± 0.30 8.98 ± 1.04 ribosomal protein S11-2 0.02 GO:0006412, GO:0003735, GO:0005840
1 2 Contig 191 -0.61 ± 0.27 10.76 ± 3.35 Unknown EST-1 0.019
2 4 Contig 212 -0.72 ± 0.38 8.77 ± 0.97 Unknown EST-2 < 0.001
4 5 Contig 229 -1.32 ± 0.11 9.96 ± 0.90 Unknown EST-3 < 0.001
4 7 Contig 249 -1.08 ± 0.27 8.13 ± 0.98 Unknown EST-4 < 0.001
1 2 Contig 56 -0.41 ± 0.36 9.25 ± 0.99 Unknown EST-5 0.056
1 1 Contig 71 -0.71 6.21 Unknown EST-6 0.027
1 1 Singlet 79 -1.08 8.90 Unknown EST-7 0.014
10 Unsequenced

aNumber of microarray elements determined to be significant on the array using the empirical Bayes method to shrink the gene-wise error estimate in cadmium treated vs. control Daphnia pulex (P < 0.05). control Daphnia pulex (P ≤ 0.05).

bNumber of microarray elements selected for sequencing based on cadmium response determined in the current study; Eads et al., in review; and Colbourne et al., in review.

cMean value (± SD) based on all sequenced microarray elements.

dPermutation tests were performed for clones sharing common putative annotations. These tests included 1000 simulations using the mean expression values to determine the Probability of obtaining the M average for an EST cluster.

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