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. 2008 Jan 10;27(2):447–457. doi: 10.1038/sj.emboj.7601976

Figure 2.

Figure 2

IpaCiv rapidly localizes at the old or new pole during division. (A) Confocal micrographs of SF621/pCiv. Red: anti-IcsA immunofluorescent staining (Materials and methods); blue: LPS staining; green: IpaCiv fluorescence. (B) Quantitative analysis of the respective localization of IpaCiv and IcsA (1304 individual bacteria, n=5). Percentages were normalized on the total number of bacteria showing a fluorescent signal for IcsA and IpaCiv. (C) Time-lapse fluorescence microscopy of SF621/pCiv growing on an agarose pad (Materials and methods). Time points are indicated in minutes. Fluorescence intensity is represented in pseudo-color code. Red and green arrows point to the formation of a fluorescent dot at the newborn or the old pole, respectively. Purple arrowheads indicate septum formation. Scale bar=5 μm.