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. 2008 Jan 10;27(2):447–457. doi: 10.1038/sj.emboj.7601976

Figure 3.

Figure 3

IpaC polarization is observed in E. coli. E. coli TOP10F' containing plasmid pBadCi and pCHAP4500 grown in the presence of 0.01 or 0.02% arabinose were incubated in the absence (−IPTG) or presence of 1 mM IPTG (+IPTG) to induce ipaC expression (Materials and methods). (A) Fluorescence microscopy analysis of representative samples grown the absence (left panels) or presence (right panels) of IPTG. (B) Extracts from cultures were analyzed by anti-IpaC (top panel) and anti-BlaM (bottom panel) western blotting. The presence or absence of IPTG, and the percentage of arabinose are indicated above each lane. The migration of IpaCi, IpaC, and the β-lactamase (BlaM) used as a control, is indicated.