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. 2008 Jan 10;27(2):447–457. doi: 10.1038/sj.emboj.7601976

Figure 5.

Figure 5

Polar IpaCic fusions inhibit type III secretion. (A) SDS–PAGE and Coomassie blue staining of proteins secreted upon Congo red induction in the supernatant of the indicated strains (Materials and methods). The expected migration of T3S substrates is mentioned. (B) Anti-GFP western blot analysis of bacterial lysates. (C) Anti-IpaD western blot analysis of bacterial pellets (P) and supernatants (S) following Congo red induction and fractionation (Materials and methods). mxiD: T3S-deficient SF401 mutant; WT: wild-type Shigella strain M90T. Full-length IpaC (C1) and the IpaCic constructs are expressed in M90T.