Figure 3.

Metabolic outcomes of single fission events. (A) Mitochondrial fission in a COS7 cell yields two daughter mitochondria with a ∼28% difference in TMRE/GFP FI ratio (∼Δ=9 mV). The relative GFP FI change was minimal (Δ=3.4%). For clarity the pseudocolored ratios before fission at 60 and 160 s after fission are shown. Scale bar, 2 μm. (B) Chart of Δψ_m over time for typical fission events; (i) fission results in temporary depolarization of one unit, (ii) fission results in permanent depolarization of one unit, (iii) fission results in the generation of a hyperpolarized daughter mitochondrion and a mitochondrion of the pre-fission potential. (C) Average Δψ_m before (left, gray) and after fission (right, filled and empty circles denote depolarized and hyperpolarized mitochondria respectively). Only fission events where Δψ_m of both mitochondria could be continuously tracked are included (n=7).