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. Author manuscript; available in PMC: 2008 Feb 7.
Published in final edited form as: J Biomol Screen. 2007 Apr 13;12(4):521–535. doi: 10.1177/1087057107300463

FIG. 4.

FIG. 4

(A) Binding of the actinonin-based fluorescence polarization probe SKI-267088 to maltose-binding protein human mitochondrial peptide deformylase (MBP-HsPDF) and test of the nonspecific binding of the probe SKI-267088 to bovine serum albumin (BSA; and cathep-sin B. The error bars represent plus or minus 1 standard deviation from triplicate measurements. (B) Displacement of the probe SKI-267088 from HsPDF by actinonin (IC50 = 2.3 μM) and the actinonin analogs SKI-267087 (IC50 = 2.1 μM), SKI-274447 (IC50 = 3.9 μM), and SKI-274448 (IC50 = 1.4 μM).