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(A) Structure of 4 compounds identified by laser nephelometry as having a solubility limit <100 μM in the fluorescence polarization (FP) assay conditions. (B) Structure of 8 compounds inducing optical interference in the FP or in the fluorescamine (FLUO) assay. The ratio of fluorescence intensity before and after addition of the compound in the assay conditions at a final concentration of 100 μM is indicated.
