Figure 6.

Uropodal adhesion depends on LFA-1 avidity regulation, and uropodal detachment is mediated by MyH9. (A–C) Human T lymphocytes were pretreated with DMSO or 50 μM blebbistatin for 1 h and allowed to migrate on ICAM-1/CXCL-12–coated surfaces for 20 min. Then cells were treated with 20 μM BIRT377, 1 μM XVA143, or an equivalent concentration of DMSO for the indicated times. The cell polarization index was then determined. Data are expressed as mean ± SEM of three experiments, each performed in triplicate. Representative DIC images from time-lapse movies are shown in C. (D) T lymphocytes were pretreated with DMSO or blebbistatin and allowed to settle on the ICAM-1/CXCL-12–coated cover glasses for 20 min in L-15 plus 2 mg/ml glucose. Cells were then treated with 20 μM BIRT377, 1 μM XVA143, or DMSO. After removal of nonadherent cells from the cover glasses, bound cells were counted. Data are expressed as mean ± SEM of three experiments.