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. 2008 Jan 21;205(1):19–24. doi: 10.1084/jem.20070201

Figure 5.

Figure 5.

HCMV-induced 5-LO expression in vascular SMCs in human tissues. (A–H) Serial sections of a blood vessel in intestinal tissue from an HCMV-infected patient with active UC were stained for the following: (A) 5-LO (FastRed, red), (B) CMV IEA (DAB, brown), (C) 5-LO/IEA double staining (FastRed/DAB), (E) SMC α-actin (DAB, brown), (F) CD31 (DAB, brown), and (G) CD68 (DAB, brown), with an area of adventitia at a higher magnification in the top right corner. (D) Image at a higher magnification of the tissue area indicated with a box in C. (H) Negative control without primary antibody showed no staining with DAB and FastRed. (I–M) Serial sections of intestinal tissue from another HCMV-infected patient with active UC stained for (I) SMC α-actin (DAB, brown), demonstrating an area with multiple small blood vessels surrounded with abundant leukocytic infiltrates. (J) Image at higher magnification of the tissue area indicated with a box in I. The adjacent sections stained for (K) 5-LO (FastRed, red) and (L) IEA (DAB, brown). (M) An HCMV− vessel in the same tissue section showed negative staining for 5-LO (FastRed, red). (N–P) Serial sections of an adrenal gland capsular vessel from one HCMV-infected AIDS patient. (N) Double staining for IEA (DAB, brown) and 5-LO (FastRed, red), showing multiple double-positive cells in the vascular wall. (O) The adjacent section stained for SMC α-actin (DAB, brown). (P) Negative control without primary antibodies showed no staining with DAB and FastRed. Bar, 50 μm.