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. 2008 Jan 21;205(1):183–193. doi: 10.1084/jem.20071022

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Copyright © 2008, The Rockefeller University Press

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Figure 2. — Comparison of antibacterial activity and antimicrobial peptide expression between neonate and adult IECs. Cationic antimicrobial peptides from 28-d-old adult (A) and 1-d-old neonate (B) mice were extracted from isolated IECs and fractionated by reverse-phase HPLC. (top) Components eluting between 10 and 50% acetonitrile (ACN). Absorbance was measured at λ = 214 nm (A₂₁₄). (bottom) Fractions were tested for antibacterial activity using B. megaterium Bm11 as the indicator strain in an agar diffusion assay. The killing zone diameter is indicated the figure. (C) Immunostaining for cryptdin 2 (red, top) and the Paneth cell marker lysozyme (red, bottom) in the small intestinal tissue of 1-, 6-, 14-, 21-, and 28-d-old mice. MFP488 phalloidin (green) and DAPI (blue) were used as counterstaining. Arrows indicate the cryptdin 2– and lysozyme-positive Paneth cells at the bottom of the intestinal crypts. Bar, 75 μm.