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. 2008 Jan 21;205(1):183–193. doi: 10.1084/jem.20071022

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Copyright © 2008, The Rockefeller University Press

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Figure 4. — Intestinal epithelial m-IC_c12 cells derived from embryonic day 20 mouse fetuses exhibit antimicrobial activity and express CRAMP. (A) Absence of enteric antimicrobial peptide expression in fetus-derived m-IC_cl2 cells measured by RT-PCR. SI, total small intestine. (B) FACS analysis and (C) immunostaining of CRAMP expression by m-IC_cl2 cells. Normal rabbit polyclonal serum was used as control. Bar, 10 μm. (D) Agar diffusion assay demonstrating antibacterial activity against the indicator strain B. megaterium Bm11 in cell lysate and supernatant of unstimulated and IL-1β– and LPS-stimulated (25 and 100 ng/ml, respectively) m-IC_cl2 cells. (E) Time course of antibacterial activity in m-IC_cl2 cell supernatant after stimulation with 100 ng/ml LPS. Activity is given as cecropin units, with one unit corresponding to the activity of 1 ng of cecropin.