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. 2008 Jan 21;205(1):233–244. doi: 10.1084/jem.20072195

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Copyright © 2008, The Rockefeller University Press

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Figure 4. — IL-10 production in co-cultures. Freshly isolated human LMNCs, LMNCs depleted of CD14⁺ cells, NK cells, and NK cells co-cultured with Kupffer cells together and separated by a transwell were stimulated by TLR agonists for 16 h. Antibodies to cell-surface CD14 and intracellular IL-10 were used to detect IL-10 expression in Kupffer cells. The results shown are from one representative donor out of six separate experiments. In the co-cultures, cytoplasmic IL-10 staining was weak in Kupffer cells stimulated with poly I:C.