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Detection of TLR expression intracellularly and on the cell surface of NK and Kupffer cells. Isolated NK and Kupffer cells were stained (open) to detect TLR on the cell surface (Perm −) or permeabilized to detect intracellular TLR. Results revealed the intracellular expression of TLR3 in both NK and Kupffer cells, whereas the expression of TLR2 and TLR4 was limited to the Kupffer cells.
