Figure 4.

Comparison of atp1 cDNA and DNA sequences in Rafflesia cantleyi. A. Agarose gel showing 1: RT-PCR results obtained using an initial reverse transcription step during thermalcycling, 2: RT-PCR results obtained without an initial reverse transcription step during thermalcycling, 3: RT-PCR results obtained without adding RNA to reaction but using an initial reverse transcription step during thermalcycling, and 4: 1 kb ladder. Results for lane 1 as compared to lane 2 indicate that cDNA was amplified from Rafflesia RNA. B. Comparisons of Rafflesia atp1 DNA and cDNA sequences. Position 931 appears to be RNA edited because a T was determined to be encoded in the cDNA while a C is encoded in the DNA.