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. 2002 Aug;120(2):147–157. doi: 10.1085/jgp.20028566

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Copyright © 2002, The Rockefeller University Press

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Figure 6. — Effect of lipid composition on the fluorescence anisotropy of DPH. The change in fluorescence anisotropy of DPH upon addition of A2E to the model membranes of DOPE, DOPS, DOPC:DOPE (1:1), DOPC:DOPE:DOPS (0.45:0.45:0.1), and DOPC:DOPE:DOPS (0.4:0.4:0.2) at 25°C was shown as a function of A2E concentration. The liposomal concentration was 100 μM (A) and 250 μM (B). The concentration of DPH in the liposomal solution was 1.5 × 10⁻⁸ M. DPH solution was injected in the membrane dispersion just before the experiment followed by gradual addition of A2E solution to reach a particular concentration of A2E.