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. 2003 Jul;122(1):115–128. doi: 10.1085/jgp.200308839

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Copyright © 2003, The Rockefeller University Press

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Figure 8. — Subcellular localization of plasma membrane Ca²⁺-ATPase in sperm. (A and B) DIC images of mouse sperm. In A, a line drawing overlays the image and arrows designate major anatomical features. (C) Sperm stained with the nuclear stain DAPI (blue) and anti-PMCA primary antibody (green). Immunoreactivity was found exclusively in the principal piece of the flagellum (arrow). Bars, 3 μm. (D) Western immunoblots indicate a major immunoreactive protein band migrating near that of a 140-kD marker protein (arrow).