TABLE III.

Effect of the Inhibition of CDK4,6–Cyclin D Complexes on the Osmotic Response of System A^A

	No treatment	200 mM sucrose
(A)
Control	2.79 ± 0.99	4.85 ± 0.92*
Ad5RSV-p16	2.36 ± 0.51	4.73 ± 0.43*
(B)
Control	2.76 ± 1.05	6.75 ± 0.93**
20 μM 3-ATA	1.91 ± 0.93	4.27 ± 1.24*

^(A)CHO-K1 cells were infected with the (Ad5RSV-p16) adenoviral vector (50 m.o.i., in serum-free EMEM). 48 h after infection, cells were either kept in normal medium or treated with 200 mM sucrose for 12 h. (B) CHO-K1 cells were preincubated in EMEM alone or supplemented with 20 mM 3-ATA for 4 h and then incubated for 12 h with no treatment or in the presence of 200 mM sucrose. In all cases, results are the mean ± SEM of quadruplicate measurements made on four independent cell cultures. Statistical significance using the Student's t test: *, P < 0.05; **, P < 0.01 sucrose versus no treatment.