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. 2004 May;123(5):521–531. doi: 10.1085/jgp.200409011

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — Single-channel recordings in the presence of ATR suggest that the channel can reopen after long-lived closed or inactivated states. Raw current traces in A–C were recorded at a holding potential of +80 mV from an inside-out patch containing one homomeric (CNGA1) channel. Data were filtered at 2 kHz, and the sampling rate was 25 kHz. The lower solid line represents the zero-current level when the channel was closed. The patch was bathed in saturating (2 mM) cGMP (A), and then 200 nM ATR was added to the bath (B and C). (A) A representative portion of a control trace depicting normal channel activity in saturating cGMP; the channel spends the majority of its time open. (B) After adding ATR, the channel activity remained relatively normal for a time. (C) Sometime before the start of this sweep, the channel entered a long-lived closed state, but reopening of the channel (with ATR still in the bath) occurred near the end of the sweep. Of the 3.2 s shown, the channel was in a closed state for 2.5 s; another portion of the record for this patch showed a 6-s closure. These long-lived closed states with ATR have been documented previously (Dean et al., 2002).