Figure 1.

Cooling reversibly stimulates hENaC in Xenopus oocytes. Shown is an example of the reversible protocol (see text). (A) Decreasing temperature from 23°C stimulated conductance and capacitance (data corrected for the amiloride-insensitive conductance). (B) Expanded scale of the changes of C_m, g _Na, and temperature demonstrating a lag between bath temperature changes and the effects on hENaC. (C) Scatter plot of C_m versus g _Na demonstrating that the majority of conductance changes occurred before the bulk changes of C_m. (D) Scatter plot of bath temperature versus g _Na demonstrating that the stimulation of g _Na lags the temperature changes. (E) Scatter plot demonstrating an initial decrease of g _Na if bath temperature was decreased below 10°C. Additional examples using a sequential temperature change (see the protocol in Fig. 3) indicated the absence of a secondary stimulation of g _Na at temperatures below ∼15°C.