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. 2004 Jun;123(6):709–727. doi: 10.1085/jgp.200308983

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Copyright © 2004, The Rockefeller University Press

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Figure 5. — Representative effect of temperature on the whole cell currents and voltage activation in a hENaC-expressing oocyte. Oocytes were held at 0 mV and clamped in increments of 20 mV from −100 to +40 mV for a period of ∼1,600 ms. Note the activation of currents at negative voltages at 15°C, followed by inhibition at 4°C. Amiloride was added at the beginning and end of the experiment. Voltage activation was fit as described by Awayda (2000) to the current response to a voltage command of −100 mV (most negative current shown). Note the change in time constant with decreasing temperature, and the increase in the magnitude of activation (ΔI_V) at 15°C followed by marked inhibition at 4°C.