Figure 7.

The Mammary Glands of PHB Heterozygous Mice Showed Hyper-Proliferation after E-P Treatment

A and B, Whole mounts of inguinal mammary glands from E-P-treated wild type (WT) (A) and PHB^+/− (B) mice, respectively (LN, lymph node). C and D, Higher magnifications of regions of panels A and B, respectively. E and F, H&E-stained sections of mammary glands shown in panels A and B, respectively. Compared with the E-P-treated wild-type mouse gland, note the significant increase in alveologenesis and ductal side branching (black arrowhead) in the E-P-treated PHB^+/− mouse gland. G, Alveolar bud number per field (±sd). **, P < 0.01) in E-P-treated wild-type and PHB^+/− mouse glands. H and I, 5-Bromo-2-deoxyuridine (BrdU) incorporation in mammary gland sections. H, Luminal epithelial cells of wild-type mammary gland, whereas panel I shows the luminal epithelial cells of PHB^+/− mammary gland. J, Average percentages of mammary epithelial cells scoring positive for BrdU staining (±sd). *, P < 0.05) in E-P-treated wild-type and PHB^+/− mouse glands.