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Analysis of Chondrocyte Markers in Primary Tumor Cells
WT or Prkar1a+/− tumor osteoblasts were isolated from mouse tails and grown in chondrocyte (CHN) or osteoblast (OBL) medium and analyzed by semiquantitative RT-PCR for the mRNAs shown at left. Gapdh was used as a normalization control. Note the marked increase in the chondrocyte markers aggrecan and collagen2 in the tumor osteoblasts. Shown are representative data from replicate experiments.
