Figure 5.

Injection of Tumor Cells Can Recapitulate Tumor Formation in Immunocompromised Mice

A, WT osteoblasts injected sc over right hip (blue arrow) of NOD-SCID mice fail to produce tumors, whereas tumor osteoblasts injected over the left hip (red arrow) produce calcifying tumors; B and C hematoxylin- and eosin-stained sections of the tumors are shown at ×40 (B) and ×400 (C) magnification and demonstrate marked similarity to the initial tumor (e.g. Fig. 1); D–F, eutopic injection of tumor cells into the tail can also produce tumors in mice, shown by x-ray (D, red arrow), or by hematoxylin and eosin at ×40 (E) or ×400 (F); G, normal mouse tail for comparison. D, Intervertebral disk; T, tumor; V, vertebrae.