Table 4.

Genes Whose Expression Is Suppressed by NGF and Further Suppressed by SH2B1b

Gene Symbol	Gene Title	Unigene	C+/C−	S+/S−	R+/R−	ΔS/ΔC	ΔR/ΔC
Pola2	DNA polymerase α-subunit II	Rn.31773	0.44	0.10	0.30	0.23	0.69
Sord	Sorbitol dehydrogenase	Rn.11334	0.13	0.04	0.12	0.28	0.89
Gcgr	Glucagon receptor	Rn.11225	0.24	0.07	0.30	0.31	1.23
Prkwnk4	Protein kinase, lysine deficient 4	Rn.12652	0.49	0.17	0.82	0.34	1.66
Ga	Liver mitochondrial glutaminase	Rn.10202	0.47	0.16	0.37	0.35	0.80
Hip1	Huntingtin interacting protein 1	Rn.50506	0.47	0.17	0.59	0.35	1.24
Syt3	Synaptotagmin 3	Rn.48884	0.28	0.11	0.46	0.38	1.63
Rb2a	Retinoblastoma-related gene	Rn.11020	0.39	0.15	0.43	0.39	1.10
Cldn3	Claudin 3	Rn.4513	0.48	0.19	0.48	0.40	1.01
U91561	Pyridoxine 5-phosphate oxidase	Rn.6299	0.45	0.20	0.40	0.44	0.89
Rab3a	Ras-related small GTP binding protein 3A	Rn.44409	0.37	0.17	0.46	0.46	1.26
Pold1	DNA polymerase δ, catalytic subunit	Rn.88690	0.27	0.13	0.32	0.48	1.21

Control PC12, PC12-SH2B1β, or PC12-SH2B1β(R555E) cells were incubated with vehicle or 100 ng/ml NGF for 6 h, and gene expression profiles were assessed as described in Materials and Methods. Gene symbols, gene titles, and unigene numbers are listed for the 12 known genes whose expression in control cells is both reduced by NGF treatment to less than 50% of basal levels (C+/C− ≤ 0.5) and whose NGF-induced reduction is further reduced by at least an additional 50% in PC12-SH2B1β cells (C+/C− ≤ 0.5; ΔS/ΔC ≤ 0.5), arranged in the order of decreasing SH2B1β-dependent inhibition of gene expression (decreasing ΔS/ΔC). Thirty-four ESTs (not listed) fulfilled these criteria. C+, C−, S+, S−, R+, R−, ΔC, ΔS, and ΔR are defined in Fig. 1. The sorting criterion of the table is designated in bold. 

^aGenes represented by more than one probe set; values listed are the average of the replicates.