Figure 2.

Resistance of Androgen-Independent Prostate Cancer Cells to TNFα-Induced AR Down-Regulation and to Apoptosis

A, Unaltered AR protein levels (analyzed by Western blot) in LNCaP-derived androgen-independent C4-2 and C4-2B cells treated with TNFα (20 ng/ml). B and C, Sustained viability of C4-2 and C4-2B cells against TNFα. The cells were treated with increasing doses of TNFα for 5 d, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay determined the number of viable cells. D, Reduction in the number of viable cells for the parental LNCaP (open bars). Absent loss of viability for LNCaP-IκB-SR cells that stably express the IκB-SR (dark-filled bar). Viable cell numbers are averages of three experiments ± sd.