Figure 8.

Essential Involvement of NF-κB (Site I) and B-myb (Site II) in the Repression of the AR Promoter

A, AR promoter (−110 to +246)-driven luciferase expression in TNFα-treated parental LNCaP or LNCaP-IκB-SR stable cells, relative to the corresponding cells treated with vehicle (PBS). All values are presented as percent of the luciferase expression from the wild-type promoter in LNCaP-IκB-SR cells, for which the value was set at 100%. The NF-κB consensus indicates replacement of site I with a consensus NF-κB element. Bar graphs indicate averages from three experiments (±sd). B, Negative regulation of the TNFα/NF-κB-responsive region in a heterologous promoter context. Averages of triplicate independent experiments, each performed in duplicate (±sd). Luciferase activity in vector (pcDNA3.1)-transfected cells was set to a value of unity. Mut, Mutated; cons, consensus; WT, wild type.