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. Author manuscript; available in PMC: 2009 Jan 30.
Published in final edited form as: J Neurosci Methods. 2007 Aug 30;167(2):258–267. doi: 10.1016/j.jneumeth.2007.08.018

Fig. 1. Serotonergic neurons in dissociated cultures from DR.

Fig. 1

Cells with positive immunoreactivity to serotonin are shown. The peroxide-antiperoxidase method (A and C-E) and the indirect immunofluorescence method (B) were used. A: Low magnification micrograph, showing many serotonin-immunoreaction positive neurons. B-E: Serotonergic neurons. Polygonal neurons (B and C), a triangular neuron (D), and a fusiform neuron (E) are shown. B: A serotonin-immunoreactive neuron treated with the indirect immunofluorescence method clearly shows its thick neuronal processes, rich arborizations, and many varicosities. DR neurons from 10-day-old rats were cultured for 11 days (A), 12 days (C-E), or 16 days (B). Bar: 50 μm.