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Serotonin-immunoreactivity (Serotonin-Immun-R) vs. 8-OH-DPAT response in cultured DR neurons
| Seroronin-Immun-R (+) | Seroronin-Immun-R (−) | |
|---|---|---|
| 8-OH-DPAT response (+) | 24 neurons (*) | 2 neurons |
| 8-OH-DPAT response (−) | 3 neurons | 10 neurons |
The experiments were performed on DR nucleus cultures made from 9 to 12-day-old rats and maintained for 4−16 days. 8-OH-DPAT was applied at 1 μM for 5 sec. The indirect fluorescence immunocytochemical method was used.
