. Author manuscript; available in PMC: 2009 Jan 30.

Published in final edited form as: J Neurosci Methods. 2007 Aug 30;167(2):258–267. doi: 10.1016/j.jneumeth.2007.08.018

Table 3.

Action potential of cultured serotonergic DR neurons

Soma diameter (μm)	19.0 ± 0.7
Resting potential (mV)	−73.0 ± 2.5
Action potential height (mV)	69.2 ± 2.4
Action potential duration (ms)	1.70 ± 0.07
Amplitude of after-hyperpolarization (mV)	20.1 ± 1.8

Values are expressed as means ± S.E.M. (n = 15). Experiments were performed on cultures from 10-day-old rats and maintained for 11−14 days. These 15 neurons were likely to be serotonergic neurons, since they were hyperpolarized by the application of 8-OH-DPAT (1 μM). The duration of action potential was measured at threshold. Experiments were conducted at 33° C using a 5 mM K⁺ external solution.