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. Author manuscript; available in PMC: 2008 Feb 8.
Published in final edited form as: Mutat Res. 2007 Aug 11;649(1-2):101–113. doi: 10.1016/j.mrgentox.2007.08.004

Table 3.

MN frequency in peripheral blood and bone marrow of male B6C3F1 mice and F344 rats administered ethyl methanesulfonate by oral gavage

Mice
Microscopy Flow Cytometry
BM PB PB
Dose (mg/kg) N1 MN-RET/1000 RET2
0 5 2.40 ± 0.43 3.20 ± 0.58 2.68 ± 0.24
50 5 4.60 ± 0.73 4.00 ± 0.42 3.66 ± 0.16
100 5 12.60 ± 1.56* 9.40 ± 2.18* 9.05 ± 0.88*
200 5 23.77 ± 1.36* 16.70 ± 2.08* 17.48 ± 2.17*
300 5 40.20 ± 6.59* 33.10 ± 1.09* 40.47 ± 2.08*
Trend test P < 0.001 P < 0.001 P < 0.001
Rats
Microscopy Flow Cytometry
BM PB PB
Dose (mg/kg) N MN-RET/1000 RET
0 5 0.90 ± 0.10 0.60 ± 0.19 0.50 ± 0.10
50 5 4.40 ± 0.40* 2.20 ± 0.46* 2.04 ± 0.14*
100 5 5.40 ± 0.83* 4.40 ± 0.37* 3.62 ± 0.61*
200 5 9.70 ± 1.56* 15.91 ± 2.32*3 7.17 ± 1.18*
300 5 11.80 ± 3.71* -----4 -----4
Trend test P < 0.001 P < 0.001 P < 0.001
*

Significantly different from control (P < 0.025/4 = 0.006)

1

N = number of animals scored

2

Data presented as mean ± SEM; see Methods and Materials, Section 2.4 for analysis procedures used for microscopy-based scoring and FCM-based scoring

3

Based on 3 animals

4

Not scorable due to depletion of circulating reticulocytes